FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-ADT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-ADT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50349199
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCC2367800.021.6081834
CGATATC2371400.021.573363
GCGATAT2376900.021.5413112
ATATCCC2376950.021.4760785
CCCTCTA2388750.021.4617129
TATCCCT2385200.021.4197836
TGCGATA2424700.021.3601471
AAGAAGA1186750.021.3032554
TCCCTCT2433700.021.297558
AGAAGAG1194350.021.212035
CTGCACC1083300.021.1791159
AAGAGAC1186000.021.1693847
ATGGTTG1155150.021.1675151
GAGACAA1194900.021.1590849
ATCCCTC2429250.021.1590637
TAACGAC605150.021.1140161
AACGACC576400.021.072912
TCTGCAC1091750.021.0664948
GAAGAGA1209350.021.0526186
CGACCAG563350.021.005494
Produced by FastQC (version 0.11.5)