FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_c-PBMCpilot-ADT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_c-PBMCpilot-ADT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52304648
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAA2821950.021.4472774
GTCAAGA2821600.021.4371996
AAGAATG2823800.021.3539479
CAAGAAT2842800.021.3295678
CACGTCA2839400.021.302323
CGTCAAG2834100.021.2758925
TCAAGAA2846300.021.2612157
ACAGAAA1038000.021.142031
CCACGTC2849600.021.1278952
AGAATGT2865550.021.0658610
TCCACGT2902400.020.9658161
CAGAAAG1019800.020.820262
AAAGTTA1016650.020.8027045
TTAGAGA1027550.020.6902129
AAGTTAG1030850.020.6260536
TAGAGAT1043600.020.36673710
GAATGTG720700.020.31884811
GCCAGCG439350.020.2952692
GTTAGAG1057300.020.2567488
AGAAAGT1075150.020.2491233
Produced by FastQC (version 0.11.5)