FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib2.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib2.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815505
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG39300.017.639031
AAACGAA39000.017.5207921
CGCATAA78050.017.439081
TGAGGAG43000.017.2727721
GACACGC55550.017.2726571
CCCGGAA36750.017.2461381
TCGAGAA39100.017.245068
GGAGAAG33250.017.203023
TAACGAC43250.017.1729321
CTGGACG39150.017.1444991
ATGAGCC39550.017.1323245
CCGAAGC37850.017.0887362
GGGATAG31850.016.9230923
GAAATAG46700.016.8886643
GATGAGG37350.016.8807131
CACGGAG52300.016.8260173
GACGGCA31600.016.8133053
GAACCGA26400.016.79121410
AAAGGCG23050.016.6069289
GCGAACA38550.016.57802410
Produced by FastQC (version 0.11.5)