FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib2.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib2.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21514571
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGACG39200.017.9090671
GACGGCA35100.017.7376143
CGCATAA79600.017.6529031
CCCGGAA39500.017.6059041
ATGGGAG42900.017.3647981
AAACGAA39850.017.3132081
TGAGGAG44900.017.277511
GCTACCT77900.017.1906171
CCGAAGC43450.017.1393722
CCCAAGA44100.017.1355084
ATGAGCC42250.017.104865
CACGGAG55050.017.1041833
CGCGTGA44400.017.1003271
CGACGGC36450.017.0810642
GAAGGGC28400.017.0417886
GATGAGG40250.017.0317971
TAACGAC44500.016.9382651
CGCCAGA36700.016.9103011
GATGAGC28300.016.868724
CGGCATG50750.016.8625955
Produced by FastQC (version 0.11.5)