FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib2.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib2.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25203469
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG50750.017.6928581
ATGAGCC50450.017.464455
CTGGACG47050.017.4470941
CGCATAA96000.017.3998071
AAACGAA47750.017.2143691
CACGGAG70350.017.199223
TAACGAC53550.017.1581671
GACACGC70450.017.1500431
CGCGTGA55000.017.065941
TGAGGAG55550.017.0356331
TCGAGAA47150.017.0303978
CCGAAGC45450.016.965982
GAAATAG63400.016.7771193
GCTACCT93800.016.775561
GGGATAG41100.016.7537753
CCATGAC60350.016.750277
ACAGAAA80250.016.6874281
GACGGCA39800.016.6653483
CCCGGAA47750.016.5691181
GATGAGG46450.016.5590481
Produced by FastQC (version 0.11.5)