FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20148510
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA77200.017.8182621
CTGGACG37650.017.8002011
TCGAGAA37000.017.7779338
CACGGAG52350.017.692543
ATGGGAG41150.017.5698531
CCGAAGC37500.017.3952242
TAACGAC41600.017.3533421
GACGGCA31650.017.3428943
ATGAGCC40900.017.1853475
GCTACCT70650.017.1337781
AAACGAA37600.017.0921861
CGCGTGA44450.017.0824411
GACACGC56100.016.8893491
CCCGGAA37950.016.8765561
CCCGAAG48350.016.819791
GAAGGGC26950.016.8158656
GAAATAG49500.016.800083
CCATGAC45750.016.758057
GATGAGC28150.016.6851124
CCCAAGA38350.016.6357464
Produced by FastQC (version 0.11.5)