FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib1.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib1.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20358161
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA73000.019.7474121
CGGAGAA46150.018.0742071
CCGGAAG49650.017.9905682
AGGGCCT62650.017.8106021
GCGCAGG40000.017.681875
ATGGGAG36700.017.5408881
GATGAGG48800.017.4535141
AACGAAA55350.017.1972981
TATGAGA42350.017.012389
ATCGGCG40600.016.9129871
TCCGGGA47300.016.8437651
TCGACGG48850.016.8049031
AAACGAG24900.016.7865499
TGACTCC49550.016.7673741
CAAAAGA27050.016.7535614
GCCCAGA51150.016.7376981
TGGGAAG34150.016.7239321
GCGGAAA38700.016.6912521
GTATGAG50650.016.6134248
GAGAAGC38500.016.5720182
Produced by FastQC (version 0.11.5)