FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib1.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib1.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21411031
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA75000.019.7483371
CGGAGAA49650.018.1736161
CCGGAAG49500.017.9780242
GCGCAGG41200.017.8345285
GATGAGG51500.017.6276111
AGGGCCT68900.017.6158281
ATCGGCG45050.017.5378511
AACGAAA59450.017.5285031
TATGAGA43350.017.4574879
CCCCAAG30600.017.3623335
ATGGGAG42650.017.1315161
GCCCAGA54550.017.0858021
GGCGAGT24350.017.075454
TCCGGGA48500.017.0163441
AATGGCT59650.016.8978611
CAAGACT70750.016.7974761
AAACGAG26850.016.7966219
TCGACGG53000.016.7965221
CGGCGCA33700.016.7447623
TAACGAC49850.016.6658921
Produced by FastQC (version 0.11.5)