FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22469551
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA80850.019.7904741
CGGAGAA53100.018.6310371
AGGGCCT70050.018.0031411
CCGGAAG53300.017.9553052
GCGCAGG45500.017.7687685
AACGAAA62250.017.5188711
ATGGGAG44100.017.517431
ATCGGCG46250.017.5120871
GATGAGG52500.017.1041971
TATGAGA47650.017.0824899
GCGAAGA26950.016.89759410
TCCGGGA47600.016.8304711
GCCCAGA54650.016.6930681
CGGCGCA36200.016.6821843
GGCGAGT22950.016.631194
AAAAGAT31700.016.621025
GGCGCAG24500.016.476944
GTATGAG57500.016.4708778
TCGACGG58750.016.445931
CAGATAC80300.016.4451391
Produced by FastQC (version 0.11.5)