FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-rna-lib-1.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-rna-lib-1.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843041
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA72500.019.549221
GCGCAGG41000.018.082475
CCGGAAG46900.018.0603712
CGGAGAA46900.017.995921
AGGGCCT65800.017.9108051
AACGAAA57450.017.6217611
ATGGGAG38850.017.5894431
TATGAGA43400.017.5387699
AAAAGAT30200.017.3008865
GATGAGG47400.017.1096321
GTATGAG51950.017.0871528
ATCGGCG41650.017.0146261
AAACGAG26200.016.8774769
GCGGAAA38200.016.8229081
AGGGAAG30950.016.8108023
TGACTCC47800.016.7592681
TGTATGA52150.016.747427
TAGACGC39100.016.6824427
GAGAAGC37950.016.6093122
GCCCAGA50850.016.5980071
Produced by FastQC (version 0.11.5)