FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACACGTCTGAACTCAAGTCACATT19.090909090909092TruSeq Adapter, Index 27 (96% over 25bp)
GGGGGAGGTGACCGGCTTTGGCCACTGG19.090909090909092No Hit
TTTGGTTTCCATGCAACGTATTACTTTT19.090909090909092No Hit
GGTTCATATTCATCATTACTAGGGAAGC19.090909090909092No Hit
ACGTACAAGTGCTCATCTATTTTCAGGA19.090909090909092No Hit
CGAGGCTGTCTTTCATGCGTATACCTTG19.090909090909092No Hit
GAACACTCAGTCTCTCGTCCTCTACCTT19.090909090909092No Hit
GGTAATCAGTGGGAAATTATACTGTTAT19.090909090909092No Hit
ACTATGGGTAGCTCGCACGCAAGTCTTT19.090909090909092No Hit
AACACAACACATAAAATTTAAAATAAAA19.090909090909092No Hit
TGGAGGACAACCCTAACCCTCGGCACAA19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)