FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-BulkC7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-BulkC7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434406
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTTT157000.016.0017997
CGTGTAG1101.8189894E-1115.9991534
GTGCTTT113850.015.8350077
GAGCTTT91000.015.8344237
GTGATTT112200.015.8031817
AGGTTTT530400.015.798288
GGGATTT137500.015.7752617
CGACGTT18250.015.730776
GAGATTT93150.015.6814987
GACGTTT86000.015.667877
AGCTTTT334700.015.60697658
AGTGCTT28350.015.5583526
GGGCTTT143250.015.5490087
CAGGTTT123200.015.5439137
AAGGTTT127900.015.3941187
AGATTTT344050.015.3650698
CGTGATT24750.015.3326156
GCTTTTT1358500.015.3314979
GGCGTTT99200.015.3017027
GGAGTTT137300.015.301547
Produced by FastQC (version 0.11.5)