FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-BulkB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-BulkB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15581672
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTCC852.4738256E-916.824022
GTCACAC10100.016.66347920
CGTGTAG802.1360393E-816.499214
GAGGTTT148400.016.232437
GAGCTTT88850.016.0195457
GTGATTT106350.015.9588557
AGGTTTT496650.015.8597578
CAGTCAC10750.015.75804318
GACGTTT82350.015.6811457
GAGATTT88250.015.6797527
GGGATTT134900.015.671657
AGAGGTT34550.015.5680476
GGGGTTT234800.015.4920677
AGTGATT28000.015.477886
TGATTTT357250.015.4501158
AAGGTTT124550.015.4284557
CGGGATT31600.015.42019756
CAGGTTT109650.015.4183727
GGGGATT44250.015.4117436
TGAGGTT39400.015.4104796
Produced by FastQC (version 0.11.5)