FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-BulkA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-BulkA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17338457
Sequences flagged as poor quality0
Sequence length28
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTTT102650.015.848487
GGGGGCT18850.015.8138495
GAGGTTT164800.015.7919557
CGAGCTT24150.015.7138156
GGGCTTT157400.015.6608577
GTGCTTT126550.015.6193997
GTGATTT116500.015.597777
GAGATTT98100.015.529567
GACGTTT91650.015.4703047
AGGTTTT552050.015.4100548
GGTGCTT40150.015.3694026
GGGGGAT17500.015.3366825
CGACCTT12850.015.3224956
CCCGATT11350.015.3123176
CGGGCTT36500.015.3091316
GGGATTT142400.015.2944417
GACCTTT58150.015.2651917
GCGGTTT160600.015.2323757
CAGGTTT128000.015.1760637
CGTGCTT27500.015.1595446
Produced by FastQC (version 0.11.5)