FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-ADT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-ADT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50349199
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCC2385750.021.6240524
CGATATC2389850.021.5845573
GCGATAT2396650.021.540172
CCCTCTA2406700.021.4771449
ATATCCC2396650.021.4758055
TATCCCT2403250.021.436516
TGCGATA2445400.021.3521041
TCCCTCT2449450.021.3326158
AAGAAGA1186450.021.3068034
AGAAGAG1193050.021.2397695
ATCCCTC2445250.021.1870697
AAGAGAC1185950.021.1795717
TAACGAC608750.021.175761
CTGCACC1080300.021.1615589
ATGGTTG1152100.021.148511
GAGACAA1196800.021.1438679
AACGACC580100.021.1092852
GAAGAGA1208800.021.0740436
CGACCAG566800.021.0736564
TCTGCAC1089300.021.0381558
Produced by FastQC (version 0.11.5)