FastQCFastQC Report
Tue 4 Jun 2019
HFGFNBGXB_n01_b-PBMCpilot-ADT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGFNBGXB_n01_b-PBMCpilot-ADT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52304648
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAA2820500.021.4532554
GTCAAGA2820100.021.4423986
AAGAATG2822850.021.3595929
CAAGAAT2842750.021.3307368
CACGTCA2838700.021.3105893
CGTCAAG2833000.021.270995
TCAAGAA2847000.021.2602777
CCACGTC2849650.021.1301442
ACAGAAA1041150.021.113941
AGAATGT2867300.021.05378710
TCCACGT2901750.020.9708291
CAGAAAG1022650.020.8127122
AAAGTTA1019300.020.796145
TTAGAGA1028750.020.6981729
AAGTTAG1031600.020.6516136
TAGAGAT1044650.020.3799810
GAATGTG724150.020.3313511
AGAAAGT1075900.020.2799113
GCCAGCG440650.020.2702622
GTTAGAG1058700.020.2569948
Produced by FastQC (version 0.11.5)