FastQCFastQC Report
Sat 13 Feb 2021
HFGF5BGXH_n02_10X_CD8_TCR_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGF5BGXH_n02_10X_CD8_TCR_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7826920
Sequences flagged as poor quality0
Sequence length49
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGT4067015.196181895304922No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1583822.02355460385439No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGT1471061.8794877167519282No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC589660.7533742519407378No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC182060.2326074624501081No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTT172160.21995880882901575No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT141870.18125903931559287No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTT131810.1684059630097152No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84770.10830569368282798No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTG78640.10047374957199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)