FastQCFastQC Report
Sat 13 Feb 2021
HFGF5BGXH_n02_10X_CD8_TCR_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGF5BGXH_n02_10X_CD8_TCR_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8130726
Sequences flagged as poor quality0
Sequence length49
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGT4185595.147867484404222No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1647762.0265840959343606No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGT1497851.842209416477692No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC608150.7479651878565333No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC188920.23235317485794013No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTT172740.21245335287402378No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT147350.18122612913041222No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTT132090.1624578174199942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84670.10413584223598238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)