FastQCFastQC Report
Sat 13 Feb 2021
HFGF5BGXH_n02_10X_CD8_ORF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGF5BGXH_n02_10X_CD8_ORF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31483622
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT12751464.050188380485575No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC10777223.4231194873321753No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3204121.017710097014886No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA2851590.905737592707726No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA2684740.8527417842839048No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC2505690.7958709452171672No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG2410160.7655281847812808No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2227160.7074027251375334No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT2164940.6876400688586592No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG978500.3107965150896552No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT801780.25466574335062214Clontech SMART CDS Primer II A (100% over 26bp)
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG786850.2499235951949874No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA786140.24969808111658817No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC762290.24212271383514897No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG753350.23928314220009375No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT739710.23495073089112808No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG659160.20936599988400317No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT646730.2054179153847038No Hit
GTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGATCC508180.16141090755059884No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT463820.14732104203258445No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA427700.13584841032585135No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT350460.1113150195997144No Hit
CTGGGGACTGCCCTTCCAGAAGAGCCGCTCCAGCTCAAAGGCTCCAGGG338620.10755433412331021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)