FastQCFastQC Report
Sat 13 Feb 2021
HFGF5BGXH_n02_10X_CD8_ORF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGF5BGXH_n02_10X_CD8_ORF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33611400
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT13648204.060586586693801No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC11555473.4379615249587934No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3443381.024467888871038No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA2995290.8911530016601511No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA2874220.8551324848117008No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCAC2631040.7827820322866645No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAG2582080.768215545915969No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG2409380.7168341693592055No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAAT2352840.700012495760367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1029970.3064347215528065No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT876940.2609055261012633Clontech SMART CDS Primer II A (100% over 26bp)
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG842960.2507958609281375No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA842210.250572722350155No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG820240.2440362496057885No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC803180.23896059075194728No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTT786740.2340693931225715No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGG709400.21105934296101916No Hit
CCGTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGAT687500.20454369648393103No Hit
GTGGGCTTGTACTCACGCATGATGTGATCTGTTTAAATACTGACGATCC535210.1592346644293305No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACT507240.15091308306110426No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGA459250.13663518925126594No Hit
GGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT374990.11156631381019536No Hit
CTGGGGACTGCCCTTCCAGAAGAGCCGCTCCAGCTCAAAGGCTCCAGGG361580.10757659603586878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)