FastQCFastQC Report
Fri 6 Sep 2019
HFGCCBGXC_n01_sgRNAseq_chr_rem_com_08302019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFGCCBGXC_n01_sgRNAseq_chr_rem_com_08302019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260175276
Sequences flagged as poor quality0
Sequence length162
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18501310.7111094599165525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG4492100.0151.964137
TCCGCCG3180050.0150.810857
CTTCGCC924500.0150.269076
GTACGCC43328100.0148.959921
AGGCGCC778250.0147.086656
ATCCGCC798200.0146.91756
CGTCGCC663850.0145.41766
GTTCGCC1054650.0145.247966
GTCCGCC818800.0144.859256
CCTCGCC634100.0144.443686
TTACGCC1371300.0144.058076
ACTCGCC786900.0143.121266
CCACGCC552350.0142.768366
ATTACGC356150.0141.438255
ACCCGCC544000.0141.333256
CTCGCCG3182200.0141.082767
CTACGCC883900.0140.418526
TTTTCGC538100.0140.318285
CTTACGC278700.0139.502725
CCTTCGC200100.0137.989535
Produced by FastQC (version 0.11.5)