FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17280804
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3214841.8603532567118983TruSeq Adapter, Index 3 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG111950.044.3840561
GCTTGAA711300.039.96536360
AAAAAAG708450.038.8746369
GAAAAAA817450.038.25322364
TGCTTGA775100.035.95023359
CTTGAAA799150.035.92167761
CTGCTTG798400.034.2961458
TGAAAAA924950.033.68425863
TTGAAAA901050.032.38765762
CGTGTGC154150.032.138572
GACGTGT38150.029.485711
GCCGTCT997500.028.59545150
GTGTGCT174250.028.431343
TCTGCTT975750.028.42672557
TGCCGTC1049650.028.21005649
CCGTCTT1027500.028.18024451
ATGCCGT1070350.027.9965648
CTTCTGC994650.027.9628855
TATGCCG1092750.027.71311847
CGTCTTC1029650.027.45128652
Produced by FastQC (version 0.11.2)