FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15743609
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3887392.469186067819647RNA PCR Primer, Index 24 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG77850.042.995521
AAAAAAG764250.041.76033869
GAAAAAA921350.039.48879664
GCTTGAA1076950.031.57300660
TGCTTGA1111600.030.15743659
CTGCTTG1112000.029.72465558
CTTGAAA1175700.029.15879261
CGTGTGC116750.028.6343522
GAGATCG213950.028.1874056
GACGTGT27750.027.8544851
TGAAAAA1309100.027.70541263
TTGAAAA1257050.027.61209762
GCCGTCT1255100.027.25917850
TGCCGTC1296600.027.01197649
CCGTCTT1280300.026.98940851
ATGCCGT1316700.026.78041648
TCTGCTT1247600.026.72896857
CGTCTTC1273300.026.59318552
CTTCTGC1257950.026.55293355
TATGCCG1337650.026.53120847
Produced by FastQC (version 0.11.2)