FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16081583
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG2517541.5654802142301538TruSeq Adapter, Index 1 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA271550.16885775486157054TruSeq Adapter, Index 1 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGG208020.12935293745646806TruSeq Adapter, Index 1 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA603650.037.21673260
TGCTTGA633850.035.35099859
CTGCTTG638350.034.98830458
AAAAAAG586500.032.6933669
GCCGTCT693100.032.36404450
CTTGAAA703550.032.13321761
GAAAAAA716750.032.00832464
TGCCGTC704200.031.89309549
TATGCCG711100.031.6175947
ATGCCGT711850.031.60366448
CCGTCTT715700.031.42391651
ACGTGTG43850.031.3193681
GTATGCC725000.031.03043746
CGTATGC755050.029.85934445
GATCTCG727650.029.8460140
CGTCTTC757050.029.75767352
TCTGCTT753150.029.75593257
CTTCTGC760900.029.6024855
CTCGTAT745900.029.48550643
TGAAAAA780600.029.24874563
Produced by FastQC (version 0.11.2)