FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_778h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_778h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14463979
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG65500.047.9938281
GTCACGG18100.013.72410829
CACGGCT18600.012.61296531
TCACGGC20000.011.73013730
AGTCACG22050.011.57864828
GCACACG21350.010.82678811
GGCCCCC26200.010.00747437
CGGCTAC25350.09.79879633
CACACGT25900.09.32443312
ACGTCTG28000.09.11790115
GGGGGCC27150.09.02204934
ACACGTC27650.08.98370613
ACGGCTA27100.08.91148232
GCCCCCC37000.08.67148338
GCCGTCT27150.08.64071749
GGGCCCC29000.08.56538136
CATCTCG28700.08.29435139
CGTCTGA31300.07.93605816
CAGTCAC35450.07.88324427
GATCGGA29850.07.86091041
Produced by FastQC (version 0.11.2)