FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_778h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_778h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11662395
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG78450.038.8382451
GCACACG21950.028.92015811
ACACGTC24250.025.75041213
CACACGT25800.024.7383812
ACGTCTG26500.023.6944515
CACGTCT26650.023.56098214
AGCACAC30550.020.89189710
GAGCACA31700.020.3516549
GAACTCC32250.019.8987921
GTATGCC26950.018.68985245
CAGTCAC34700.018.49383227
CACTAGC34450.018.12650731
TATCTCG26800.018.02192939
CGTCTGA35650.018.00000616
TGCCGTC27600.017.7495548
TCCAGTC35700.017.68601625
AGTCACT38900.016.94046628
ATGCCGT29700.016.14605547
TAGCTTA40250.016.02850734
CCAGTCA40100.015.83137926
Produced by FastQC (version 0.11.2)