FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_777h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_777h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12223579
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG45150.047.9183851
GCACACG19250.017.9221711
AGTCACG20150.016.26639228
CGATCAG21500.016.2070633
GTCACGA22450.015.21438529
GCCGTCT24100.014.45810749
ACACGTC24800.014.18947313
CACACGT24800.014.05053312
GATCGGA23600.013.7438451
TATGCCG27600.012.62475546
CACGATC27000.012.52233731
CACGTCT29050.012.469995514
ACGTCTG28600.012.3043615
GTATGCC30350.011.59455145
CGTCTGA29150.011.48038116
GATCTCG29100.011.26280639
TGCCGTC33150.010.71916248
AGCACAC33800.010.41125310
CGTATGC33800.010.30901344
CTCGTAT35000.010.15274342
Produced by FastQC (version 0.11.2)