FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_777h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_777h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16417000
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG141350.050.141531
GCACACG25550.013.2328311
GTCACAC24150.012.57203529
CACACGT26250.012.48570812
ACGTCTG27050.012.37160315
ACACGTC26100.012.2930613
CACGTCT27350.011.98357814
GGCCCCC55550.010.49581737
GGGGCCC51250.09.96280135
CGTCTGA36850.09.73673616
GGGCCCC58400.09.5110136
GGGGGCC51050.09.39381234
AGCACAC37750.09.32182210
GAACTCC37100.09.11345421
GAGCACA35850.08.94975959
GCGGGGC12150.08.51864433
AGTCACA41450.07.824309328
TCCAGTC44150.07.73642925
GCCCCCC78250.07.583290638
CAGTCAC45800.07.53306927
Produced by FastQC (version 0.11.2)