FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15868062
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG440160.27738737093414434TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG180450.028.8878258
GCCGTCT185500.028.58492950
TGCCGTC197000.028.1421249
TATGCCG209350.027.63558247
ATGCCGT210100.027.09358448
TGCTTGA198600.026.73416159
GCTTGAA206800.026.4414960
ACGTGTG36100.026.285911
CCGTCTT209500.026.00191351
GTATGCC225550.025.92599746
GATCTCG159550.025.01764940
CGTATGC234000.024.75388545
TCGTATG241650.023.1707644
AGTGATC182550.023.16974837
CTCGTAT170350.023.14795543
CGTGTGC42300.022.5102332
GTGATCT181050.022.40894538
ATCTCGT177950.022.39205441
CGTCTTC242200.022.07825552
CTTCTGC253650.021.16322755
Produced by FastQC (version 0.11.2)