FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_773h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_773h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11322404
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG136800.058.796651
GCACACG21000.029.07912411
AGTCACG21450.028.14714228
GTCACGT22900.026.21424129
CACACGT23450.026.18813312
ACACGTC25100.024.60394513
GATCGGA25850.023.6270311
CGTTTCG27150.022.36457433
ACGTCTG29650.021.0611115
CACGTCT30650.020.03627214
CGTCTGA30650.019.92326716
GTTTCGG30750.019.29750434
AGCACAC33100.018.76160410
GAACTCC37200.016.78646321
GAGCACA39100.016.5884069
TCCAGTC38150.016.00685925
CAGTCAC38400.015.81273227
AGAGCAC42450.015.0352248
CCAGTCA41900.014.65660426
TCGGAAG47000.013.8734863
Produced by FastQC (version 0.11.2)