FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_773h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_773h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13721801
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG24150.040.57225431
AGTCACG25000.038.36562328
CGTGGCC26600.036.44556833
ACGTGGC26950.036.22867632
AGGGGGG91500.036.089341
TCACGTG27400.035.76011330
GTCACGT27100.035.64687329
GCACACG28550.034.44015511
CACACGT29650.033.39540512
ACACGTC31500.031.3242213
ACGTCTG32250.030.59597415
TATCTCG32900.029.36144641
CACGTCT35000.028.09343114
GTATGCC34900.027.57968547
AGCACAC37000.026.85450210
TGCCGTC35850.026.75251250
GGCCTTA36350.026.47982236
GAACTCC39700.025.20202421
ATGCCGT38300.025.04119149
CAGTCAC39350.024.72540527
Produced by FastQC (version 0.11.2)