FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17525954
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG433180.24716486189567768TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG163900.023.70104858
GCCGTCT169450.023.06728650
TGCCGTC173350.022.5682249
TGCTTGA176200.022.26192359
TATGCCG181700.021.56907547
GTATGCC185900.021.21185746
GCTTGAA187800.021.18077560
ATGCCGT186200.021.10338648
CCGTCTT189850.020.75217451
CGTATGC206750.019.03934545
CTCGTAT208250.018.23960343
ATCTCGT211500.017.8288341
TCGTATG226950.017.43592844
AATCTCG219300.017.16323740
CGTCTTC239600.016.73121552
CGTGTGC32700.016.4585822
TCTCGTA235750.016.3314842
ACTGACC243750.016.2909933
ACGTGTG33150.016.030861
CTTCTGC250950.016.00198755
Produced by FastQC (version 0.11.2)