FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18266232
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG848540.46454025110378544TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG321100.023.6799158
GCCGTCT329400.023.33460250
TGCCGTC333400.023.23055549
TGCTTGA333300.022.947759
TATGCCG341250.022.62539547
GTATGCC342500.022.56309346
GCTTGAA346950.022.19402560
ATGCCGT350250.022.10311748
CCGTCTT350450.021.95268251
TATCTCG359350.021.30349340
GTCACGC371750.020.85371830
CTCGTAT369100.020.79683143
CGTATGC372500.020.76445445
TCACGCC376100.020.66727631
AGTCACG381900.020.36304729
CACGCCA383150.020.31395532
ATCTCGT387350.019.81698641
TCGTATG393650.019.68387844
ACGCCAA402500.019.38864133
CGTCTTC402850.019.19999152
Produced by FastQC (version 0.11.2)