FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_770h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_770h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12822173
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG296300.060.767671
CACGAGT24450.027.79753131
AGTCACG26150.025.7280728
ACGAGTG26750.025.27829432
GCACACG26650.024.98468611
CGAGTGG27600.024.62450633
GTCACGA29200.023.0404529
CACACGT31850.021.33891112
GCCGTCT31850.021.33857751
TCACGAG32400.021.19046630
ACACGTC34100.020.03209113
GATCGGA33950.019.413051
GTATGCC37650.018.32625647
TATGCCG37700.018.30194948
TATCTCG37300.018.22095741
CGTCTGA37800.018.1626916
ACGTCTG38150.018.0864915
CACGTCT37600.017.98396314
TGCCGTC38900.017.55999250
AGCACAC40000.016.99104310
Produced by FastQC (version 0.11.2)