FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_770h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_770h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237784
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA190100.14360409567039317TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG154800.054.8359341
CCGTACG38200.049.6722133
CGTACGT43250.043.87233434
GCACACG45150.042.1798811
CACACGT47350.040.5845612
ACACGTC47300.040.55421413
ACGTCTG47950.039.71711715
CACGTCT48850.038.98538214
GCCGTCT49800.038.1708251
TGCCGTC51600.036.8392850
GTACGTA52300.036.08276435
AGCACAC54750.035.414110
GTATGCC54700.034.24693747
CGTCTGA57550.033.45116416
GATCGGA56900.032.990411
ATGCCGT57500.032.87925349
GAGCACA59150.032.779629
TCCAGTC59350.032.6706425
GAACTCC59950.032.51569721
CAGTCAC59800.032.19376827
Produced by FastQC (version 0.11.2)