FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_769h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_769h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12210213
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG103600.056.3560261
GCACACG22900.025.15942411
CACACGT26250.021.81717312
GATCGGA26550.020.7948231
ACACGTC29150.020.35680413
GCCGTCT27850.019.69629551
TATGCCG30150.018.4226148
ACGTCTG31800.018.33515715
CACGTCT32200.018.2144614
CGTCTGA32650.017.54060716
TATCTCG33450.016.91461641
CGTATGC34350.016.67230846
AGCACAC35300.016.51702110
CAGTCAC37350.016.16514627
ATGCCGT36100.016.15072849
GTATGCC36250.015.70321447
TGCCGTC36550.015.66871350
GTCACAT40500.015.07810929
GAGCACA38800.014.6714129
AGTCACA41700.014.39611728
Produced by FastQC (version 0.11.2)