FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_769h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_769h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13710057
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG171100.050.3583681
GTCACAC23700.021.98205829
GCACACG25600.020.48452811
ACACGTC26100.019.82758913
CACACGT29200.017.8409512
TATCTCG28950.017.7560941
ACGTCTG30800.017.47421815
CACGTCT30150.017.393114
GTATGCC30400.017.36310847
GCCGTCT34500.015.59960551
TGCCGTC34100.015.5802550
AGCACAC36750.014.83276310
GAGCACA37700.014.2759699
ATGCCGT37300.014.05862349
AGTCACA38300.014.053040528
GATCGGA36300.013.9737761
GAACTCC38650.013.8360121
TCCAGTC39950.013.386312525
CGTCTGA41900.013.09183916
CGTATGC41700.012.98892646
Produced by FastQC (version 0.11.2)