FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24202
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1780.73547640690852No Hit
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA1520.6280472688207586TruSeq Adapter, Index 22 (97% over 37bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1510.6239153788943063No Hit
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT1450.5991240393355921TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA1060.43798033220395005TruSeq Adapter, Index 22 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG870.35947442360135523TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA700.2892322948516652RNA PCR Primer, Index 24 (100% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA620.25617717544004626TruSeq Adapter, Index 14 (97% over 38bp)
AACAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC610.25204528551359395TruSeq Adapter, Index 22 (97% over 37bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC410.16940748698454675TruSeq Adapter, Index 22 (97% over 37bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT390.161143707131642TruSeq Adapter, Index 22 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC380.15701181720518964TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAT290.11982480786711842TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA260.10742913808776135TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG900.069.01
AACTCAG253.003841E-669.023
ACTCAGT253.003841E-669.024
TCTAATC150.002327714669.047
CTCAGTC253.003841E-669.025
CGATCTA208.3472165E-569.014
TTAGATC150.002327714669.010
TCAGTCA353.153782E-759.14285326
GAACTCA308.860194E-657.522
GTCAATC200.00727275451.75000462
TCAATCT200.00727275451.75000463
GTGTGCT552.421075E-950.1818163
TGTGCTC552.421075E-950.1818164
ACGTGTG552.421075E-950.1818161
GTGCTCT552.421075E-950.1818165
CCCACAG451.7865023E-646.036
CACCCAC451.7865023E-646.034
TCCACCC451.7865023E-646.032
CGTGTGC605.2295945E-946.02
ACTCCAC551.3915997E-743.9090930
Produced by FastQC (version 0.11.2)