FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_734h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_734h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134312
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG192400.059.398821
ACGTCCG12300.023.84174732
CGTCCGC13950.021.26878533
AGTCACG18950.016.0216628
GTCACGT21050.015.07890329
GCACACG20450.015.01505111
GTCCGCA22650.014.01309734
CACACGT22700.013.83074812
CACGTCC23300.013.17838831
GGCCCCC74600.012.99511237
CCGCACA22750.012.88991536
GATCGGA25550.012.6949061
ACACGTC25750.012.19226913
GCCGTCT25800.012.16836751
TCACGTC26100.012.02904130
GGGGCCC64600.011.85585835
CATCTCG25100.011.820538541
GGGCCCC69500.011.8142136
CACGTCT28450.011.76277514
CGTCTGA28250.011.35730116
Produced by FastQC (version 0.11.2)