FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_734h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_734h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16021582
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5217713.2566759012936424TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1864961.1640298692101692TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240860.15033471725825828No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217220.13557962004001853No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT203770.12718469374622304TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG820900.066.78631611
ACACGTC820650.066.7679913
CACACGT821900.066.7094612
ACGTCTG820350.066.63763415
CACGTCT823750.066.4124314
AGCACAC832900.065.89036610
GATCGGA814950.065.7613751
GAACTCC825700.065.7512521
AGTCACA828050.065.64107528
GAGCACA837400.065.626929
CGTCTGA834350.065.52672616
GTCTGAA834800.065.51617
GTATGCC823200.065.5071947
GTCACAG830300.065.4006629
TCCAGTC828800.065.3737425
GCCGTCT822750.065.2578751
TGCCGTC826650.065.1044250
CAGTCAC836800.064.92975627
TCGGAAG849050.064.850993
ATGCCGT831650.064.833349
Produced by FastQC (version 0.11.2)