FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_663h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_663h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14661697
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA504530.3441143272842154TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362760.2474201997217648No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG287610.19616419572713856TruSeq Adapter, Index 16 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161980.1104783436733142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1348600.066.071121
CGTCCCG107050.056.88248435
GTCCCGA108950.055.54179436
GATCGGA116700.053.87441
ACCCGTC115100.053.2046432
TCACCCG115500.053.1409530
GCACACG118750.052.61449411
CCCGTCC116600.052.54959533
CCCGATC111700.052.35209738
CACACGT120500.051.8505512
ACACGTC122450.050.99614713
TCCCGAT119050.050.7427937
CACCCGT121050.050.5896331
CCGATCT117450.049.8181339
CGATCTC118500.049.3763740
GATCTCG119250.049.12368441
CACGTCT128000.048.6773414
ACGTCTG128650.048.53883415
GCCGTCT127650.047.9718251
CGTCTGA131050.047.64958216
Produced by FastQC (version 0.11.2)