FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_663h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_663h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11290239
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG368980.3268132764948554TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG298250.058.1128651
GCACACG70800.054.625411
CACACGT73100.053.00130512
ACACGTC73300.052.90352613
CACGTCT75000.051.70437214
ACGTCTG76400.050.57650815
AGCACAC78250.049.68917510
GTATGCC76100.049.4598445
GAACTCC79550.048.6175921
CAGTCAC79950.048.4617327
TCCAGTC81400.047.68324325
TGCCGTC79300.047.50728248
GAGCACA81950.047.487849
ATGCCGT80300.047.17343547
GATCGGA81300.046.983641
AGTCACC86250.045.16172828
CCTTGTA85700.045.127933
TCGGAAG88100.044.563863
CGTCTGA87800.044.24480416
CCAGTCA89000.043.88257626
Produced by FastQC (version 0.11.2)