FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19177969
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG361760.18863311333958252TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG154850.021.03145258
ACGTGTG33000.020.704841
TGCTTGA171900.019.80841859
GCCGTCT181800.019.03336350
TGCCGTC191050.018.85219849
GCTTGAA190050.018.58833960
TATGCCG204750.018.04571347
ATGCCGT204650.017.73423848
CGTGTGC38250.017.5881292
GTATGCC214700.017.386346
CCGTCTT205800.017.26634251
CGTATGC229700.016.04065745
TATCTCG240850.015.81389640
CTCGTAT239000.015.43091743
TCGTATG241900.014.97494844
ATCTCGT260050.014.52692541
CGTCTTC240350.014.138411552
GTGTGCT50250.013.8685713
CCGATGT302150.013.80455534
CGATGTA309450.013.53468235
Produced by FastQC (version 0.11.2)