FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_323h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_323h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27317759
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1312380.48041276006571404TruSeq Adapter, Index 15 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG334800.058.64591
GCTTGAA417400.028.00265760
GCCGTCT429200.027.01580250
CTGCTTG430450.026.72090558
TATGCCG444650.026.24008247
TGCTTGA448150.025.90418459
ATGCCGT452150.025.83534648
GTATGCC454900.025.77789746
TGCCGTC451450.025.77601449
CCGTCTT473900.024.5622151
CGTATGC484800.024.25204845
CTCGTAT493250.022.92732243
ATCTCGT504500.022.40930241
TCGTATG523650.022.38688944
TCTCGTA509800.022.25068742
ACGTGTG65700.022.16451
AATCTCG515600.021.86668840
GTCAATC519150.021.59097737
GCACACG667900.021.13192412
GAACTCC669650.020.88153522
Produced by FastQC (version 0.11.2)