FastQCFastQC Report
Wed 10 Jan 2018
HFG2NBGX5_n01_323h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFG2NBGX5_n01_323h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12211049
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG459750.061.6216431
CATCTCG27700.032.63125639
ACACGTC33100.031.58159313
GCACACG33600.031.1116311
CACACGT34200.030.66681312
ACGTCTG35100.029.97877315
CACGTCT37500.028.24413514
GTATGCC30950.028.08991245
GAACTCC39900.026.2862721
CGTCTGA40600.025.74734316
AGCACAC40450.025.67245110
TGCCGTC34250.025.48416548
GAGCACA42100.024.9940789
CAGTCAC43200.024.3590527
GCCGTCT34500.023.59953149
TCCAGTC46550.022.97643925
CCAGATC47550.022.12935333
AGTCACC48450.022.07540328
CCAGTCA48500.021.76818326
ATGCCGT39550.021.45848847
Produced by FastQC (version 0.11.2)