FastQCFastQC Report
Thu 21 Dec 2017
HFFVNBGX5_n02_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVNBGX5_n02_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34369940
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1574880.4582143582444426No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT1123830.32698049516525196No Hit
CAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTCACATGGAAAGCC828220.24097219838032885No Hit
GTAGTAGTATTCTAAGAATATACATATTTCTTTCTCACATGGAAAGCCTC790050.22986656363089375No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT710950.20685226683549635No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT676350.19678533043700394No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGG621160.18072769402565148No Hit
TTATTACACACACAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTC473780.13784720019877836No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC454920.13235984700584288No Hit
GTGTATGGAGTGTTCAATTTCTAGTATGAGAAATTAACGATTTAAGTCCT427730.12444886432737444No Hit
TACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGT415730.12095744129899558No Hit
ATATACTTGTATGTTATTACACACACAGTAGTAGTATTCTAAGAATATAC358260.10423643451225112No Hit
ATTACACACACAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTCAC357370.10397748730431303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.030.3150751
GTATCAA537200.029.1265371
TAGATCT679700.028.86206244
GTAGATC787500.024.84401143
TCTCGGT98500.021.28543744
ATCTCGG164550.018.59752544
TAAGTCC103150.018.5555643
GAGTGAA2657100.017.2757971
AGTGAAT2688500.017.0307352
CCGCGGG11900.016.82365444
GATCTCG185300.016.728643
TCAACGC972400.016.4914323
TGTAGAT1189200.016.46117642
CTAGTAT120800.016.37189921
TATCAAC985200.016.3510421
ATCAACG990650.016.1378822
TAGTATG125800.015.63377422
AAGTCCT126650.015.3905644
CAACGCA1069050.015.0557874
GTATGAG130650.014.91868724
Produced by FastQC (version 0.11.2)