FastQCFastQC Report
Wed 20 Dec 2017
HFFVNBGX5_n02_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVNBGX5_n02_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32282260
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT885820.27439838474753625No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT612740.1898070333365756No Hit
GAGTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGA547430.16957610774462506No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT509300.1577646670338446No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGG464240.14380653646925587No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATC391420.12124925578320725No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT384820.11920478925577083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG125650.027.1189611
TAGATCT425350.026.38363644
GTATCAA290950.025.1103941
GTAGATC497750.022.5106243
GGTATCA96400.022.4689431
AGTACGT168300.020.7872622
TCTCGGT71600.018.34370644
CGGTTCA86600.016.94408834
TCAACGC484950.016.6542843
GATCTCG135550.015.79204543
GAGTGAA1233400.015.3839521
ATCAACG523450.015.3427062
TGTAGAT746350.015.11573342
AGTGAAT1262750.014.9974942
TATACAG159500.014.9791375
TCGGTTC98750.014.97078133
TATCAAC537700.014.9341121
CAACGCA547650.014.7832684
CTTATAC148700.014.3219763
GGTTCAA107250.013.80473435
Produced by FastQC (version 0.11.2)