FastQCFastQC Report
Wed 20 Dec 2017
HFFVNBGX5_n01_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVNBGX5_n01_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34369940
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3418700.9946773255932364No Hit
GTCCTGTCTCTTATACACAT1464570.4261194520560699No Hit
GTGCTGTCTCTTATACACAT1130890.32903461571361486No Hit
GTACTGTCTCTTATACACAT681160.1981848091675458No Hit
GTTCTGTCTCTTATACACAT670450.19506871411471768No Hit
CTGTCTCTTATACACATCTC610860.1777308892596263No Hit
GTACCTGTCTCTTATACACA435890.12682303198667208No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC46300.012.0990291
GTACTCC56250.012.0750991
GTACTCA57600.011.865031
GTCCTAC59150.011.8145531
GTCCCAA87450.011.7065531
GTACTGC76350.011.6842781
GTACTGT130500.011.5953991
GTACTCT44650.011.4013011
GTGCCCC93350.011.394231
GTCTCAC64200.011.3869381
GTACTAT53700.011.3575691
GTGCACC65200.011.351911
GTCGTAC40250.011.32547951
GTACCCC77250.011.2580871
GTACTTA45850.011.2098081
GTCTCCA71700.011.1529161
GTACCCA68150.011.1071171
GTGCAAC60450.011.0855471
GTACCCT88700.011.0442331
GTGACCA58700.011.0223791
Produced by FastQC (version 0.11.2)