FastQCFastQC Report
Wed 20 Dec 2017
HFFVNBGX5_n01_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVNBGX5_n01_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32282260
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3038490.9412259240833821No Hit
GTCCTGTCTCTTATACACAT822840.254889217793302No Hit
GTGCTGTCTCTTATACACAT616060.19083546195340723No Hit
GTACTCCTGTCTCTTATACA531240.16456096939929235No Hit
GTACTGTCTCTTATACACAT489020.15148257897681267No Hit
GTAAGCAGTGGTATCAACGC475700.14735647380325914No Hit
CTGTCTCTTATACACATCTC454130.14067478547041004No Hit
GTTCTGTCTCTTATACACAT382380.11844895617593068No Hit
GTACCTGTCTCTTATACACA376030.11648193156241231No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC371900.013.7491733
TATACCA349200.013.719742
ATATCAA257600.013.6924256
GTATACC368850.013.6652051
GGGCCAA620400.013.6576354
GCCAAGT623300.013.6030546
GTTTTCA269350.013.5578691
TTTTCAA259050.013.5518152
CGGGCCA581550.013.5493393
CAAGTGA627050.013.5358178
TACCACA382500.013.4797794
ACCACAC380850.013.4683385
CCAAGTG640150.013.3692557
TTCAATA269050.013.3256714
TCGGGCC557000.013.3255042
AAAAACT112200.013.3128388
TATATCA157000.013.2995255
GAACTTC180400.013.2932976
CCACACG390050.013.2924166
GAAGAAT163600.013.2678635
Produced by FastQC (version 0.11.2)