FastQCFastQC Report
Tue 17 Oct 2023
HFFVKDRX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFFVKDRX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34188128
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAT15916604.6555927250535625No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATT15187294.442270135410748No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTA14334334.192780020011625No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTT14006474.096881233157896No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTT8834852.584186533992151No Hit
TCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTA8515102.4906599156291915No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCT7728722.2606443967917755No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTT6171351.8051149217646545No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCC5249721.5355388864812956No Hit
CGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATG691710.2023246198212432No Hit
CAAACCAAAACGTTCCAAATTTTAAAATTTAAAAACGAAAAACCATAATT657250.1922450974794525No Hit
ACAAACCAAAACGTTCCAAATTTTAAAATTTAAAAACGAAAAACCATAAT641040.187503685489887No Hit
AAACATACTACTAGCAACAAATTAAACAAGATAAAAAACTACAATAATTA507770.1485223174547609No Hit
AACAAACCAAAACGTTCCAAATTTTAAAATTTAAAAACGAAAAACCATAA437830.12806492358984967No Hit
GATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTG437550.12798302381458265No Hit
TCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTAG420380.12296081259553025No Hit
TCCAACACACTCCCACAAAATAAATAAAATAAATAACTAACAATAATTAA419800.12279116306104856No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTC388060.11350723853613745No Hit
ACAAACATACTACTAGCAACAAATTAAACAAGATAAAAAACTACAATAAT353320.10334581641907974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)